What is TAE Buffer and its purpose in molecular biology?

TAE Buffer, which stands for Tris-Acetate-EDTA, is a buffer solution commonly used in molecular biology, particularly in DNA and RNA electrophoresis. It helps maintain a stable pH during experiments, which is crucial for preserving nucleic acid integrity. By using TAE Buffer, researchers can effectively separate genetic material, making it an essential tool in laboratories for applications like gel electrophoresis and cloning.

How should I store 50X TAE Buffer?

50X TAE Buffer should be stored at room temperature or in a cool, dark place. Protecting it from light helps prevent degradation of the Tris component, ensuring its effectiveness. Using proper storage techniques prolongs the buffer's shelf life, enabling multiple experiments over an extended period without compromising results.

Can I dilute 50X TAE Buffer for use in agarose gel electrophoresis?

Yes, 50X TAE Buffer should be diluted to 1X before use in agarose gel electrophoresis. To prepare a 1X solution, you can mix one part of the 50X stock with 49 parts of distilled water. This dilution ensures that the buffer maintains the correct pH and ionic strength required for effective separation of nucleic acids during electrophoresis.

What are the advantages of using TAE Buffer over TBE Buffer?

TAE Buffer has several advantages over TBE Buffer, including lower conductivity, which allows for improved resolution of large DNA fragments. Additionally, TAE can be reused multiple times, making it cost-effective for researchers. Its composition also allows for better performance in applications where DNA integrity is critical, such as in Southern blotting or when preparing DNA for sequencing.

Is 50X TAE Buffer suitable for RNA gel electrophoresis?

Yes, 50X TAE Buffer is suitable for RNA gel electrophoresis, as it provides a stable environment that helps preserve RNA integrity during electrophoretic separation. Unlike some other buffers, TAE does not chelate divalent cations that might destabilize RNA. This makes it a practical choice for visualizing RNA, studying gene expression, or analyzing RNA transcripts.

What is the role of EDTA in TAE Buffer?

EDTA, or ethylenediaminetetraacetic acid, is included in TAE Buffer to chelate divalent metal ions such as magnesium and calcium. These metal ions can catalyze the degradation of nucleic acids; therefore, by binding to them, EDTA helps to protect DNA and RNA from enzymatic digestion, preserving their integrity during experiments.

Who would typically use 50X TAE Buffer?

50X TAE Buffer is mainly used by molecular biologists, genetic researchers, and educators in laboratory settings. It's invaluable for tasks like DNA isolation, restriction enzyme digestion analysis, and cloning experiments. Anyone involved in the study of genetics or forensic science would find this buffer vital in their research and practical applications.

What safety precautions should I take when using 50X TAE Buffer?

When handling 50X TAE Buffer, it’s important to wear gloves and goggles to avoid skin and eye contact, as it can cause irritation. Ensure that your work area is well-ventilated and that any spills are cleaned up promptly. Additionally, following proper waste disposal methods for chemical products will help maintain a safe laboratory environment.

Can 50X TAE Buffer be used for other applications besides electrophoresis?

Yes, 50X TAE Buffer can also be used in various applications beyond electrophoresis. It serves as a buffer for DNA ligation, restriction digestion, and PCR reactions, largely due to its ability to maintain a stable pH and ionic conditions. Its versatility makes it a go-to buffer for numerous molecular biology techniques.

Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 500 mL in Latvia?

You can conveniently purchase 50X TAE Buffer Tris-Acetate-EDTA, 500 mL from Ubuy. Ubuy offers a reliable online platform with a wide selection of laboratory supplies, making it a great option for your research needs.

Kā iepirkties 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL tiešsaistē no Ubuy?

Ir viegli iepirkties 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.

Vai 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.

Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

eCon Lab Supply Skatīt oriģinālu

50X TAE Buffer (Tris-Acetate-EDTA), 500 mL

50X TAE Buffer is used for running electrophoresis of nucleic acids in the gels, providing electrical conductivity and maintaining the pH.

eCon Lab Supply

Preces #: 68232459

50X TAE Buffer (Tris-Acetate-EDTA), 500 mL

Preces #: 68232459

€ 66

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from ASV}

Pieejamība : Noliktavā

Importēts no veikala USA

QTY:

Pasūtiet tūlīt un saņemiet to Otrdiena, Augusts 25

Secured transaction

Our Top Logistics Partners

50X TAE Buffer is used for running electrophoresis of nucleic acids in the gels, providing electrical conductivity and maintaining the pH.

U-Care garantija:

Nav

Izvēlieties plānu

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of ASV store (110-120). Recommended power converters Pērc tagad.

What Stands Out

Optimal pH Stability

This 50X TAE buffer ensures stable pH levels vital for consistent DNA and RNA electrophoresis, enhancing accuracy and reliability in experiments.

Efficient Concentration

Highly concentrated at 50X, this buffer allows for easy dilution, saving time and reducing storage space while providing sufficient supplies for multiple applications.

Quality Assured

Manufactured with high-quality chemicals, this TAE buffer guarantees purity and performance, ensuring reliable results for researchers and laboratories focusing on molecular biology.

Produkta informācija

Manufactured in an ISO 9001 and 13485 certified facility in the USA
For research use only. Not for human or animal clinical/therapeutic use.
Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
50X TAE (Tris-Acetate-EDTA) Buffer is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH
Contains 2M Tris, 1M Acetic acid with 0.05M EDTA
Lower buffering capacity than TBE buffer, hence periodic replacement is recommended during prolonged electrophoresis

Container Type	Bottle
Item Form	Gel
Item Weight	12 ounces (340.2 grams)
Volume	500 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.18
Brand	eCon Lab Supply
Item Volume	500 ml
Package Information	Bottle

Who Should Buy?

Molecular Biologists
Ideal for DNA electrophoresis and purification processes facilitating accurate analyses in molecular biology experiments.
Educational Institutions
Essential for teaching laboratories, providing students a hands-on understanding of nucleic acid separation techniques.
Research Laboratories
Beneficial for academic and industry research labs performing extensive genetic research and cloning procedures.

Non-scientific Users
Not suitable for casual or non-research users who lack the expertise in using laboratory reagents effectively.

PRODUKTA APRAKSTS

50X TAE Buffer (Tris-Acetate-EDTA), 500 mL

About This Item

Introducing 50X TAE Buffer Tris-Acetate-EDTA, 500mL, the perfect solution for your DNA electrophoresis experiments. This laboratory-grade buffer offers high purity and is ideal for research purposes, allowing you to achieve accurate and reliable results. Our Tris-Acetate-EDTA buffer contains 2M Tris, 1M Acetic acid with 0.05M EDTA, providing excellent electrical conductivity and maintaining the pH during electrophoresis.

The EDTA present in the buffer chelates divalent cations, inhibiting metal-dependent nucleases and protecting DNA from degradation during electrophoresis, for optimal results. Our Acetate-EDTA buffer may be used for both genomic and large supercoiled DNA, and as a gel preparation buffer. It is perfect for DNA fragment analysis, nucleic acid separation, DNA extraction, and genomic DNA isolation.

With a lower buffering capacity than TBE buffer, periodic replacement of the buffer during prolonged electrophoresis is recommended. However, dsDNA runs faster with TAE buffer than TBE. Our 50X concentrated TAE buffer should be diluted with molecular biology grade water to 1X before use.

It is easy to prepare and use, making it perfect for PCR analysis and gel electrophoresis applications. Choose our Molecular grade TAE buffer for reliable and accurate results in all your DNA electrophoresis experiments. Order now and experience the convenience and quality provided by 50X TAE Buffer Tris-Acetate-EDTA, 500mL.

Vai jums ir kādi jautājumi? Terzet ar mums

Klientu jautājumi un atbildes

jautājums: Kā iepirkties 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL tiešsaistē no Ubuy?

atbildi: Ir viegli iepirkties 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.
jautājums: Vai 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

atbildi: Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. 50X TAE Buffer (Tris-Acetate-EDTA), 500 mL nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.
jautājums: Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

atbildi: Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

eCon Lab Supply Buffers Editorial Review

The 50X TAE Buffer Tris-Acetate-EDTA, 500mL is a reliable and efficient solution for DNA and RNA electrophoresis. It offers a high-quality, consistent performance that meets the needs of researchers and scientists. The buffer comes in a convenient 500mL bottle, providing ample supply for multiple experiments. The TAE buffer is easy to prepare and ensures precise and accurate results when used in gel electrophoresis. Overall, this product delivers exceptional value and is highly recommended for laboratory use.

Customer Reviews & Ratings

5.0

1 klientu vērtējumi

5 zvaigzne

100%
4 zvaigzne

0%
3 zvaigzne

0%
2 zvaigzne

0%
1 zvaigzne

0%

Pārskatiet šo produktu

Dalieties savās domās ar citiem klientiem

Pros

Reliable and efficient solution for DNA and RNA electrophoresis
High-quality and consistent performance
Convenient 500mL bottle
Easy to prepare
Delivers exceptional value

Product Price History

Svarīga informācija

Ierobežojumi: attiecībā uz produktiem, kas tiek nosūtīti starptautiski, lūdzu, ņemiet vērā, ka jebkura ražotāja garantija var nebūt derīga; ražotāja servisa iespējas var nebūt pieejamas; produktu rokasgrāmatas, instrukcijas un drošības brīdinājumi var nebūt galamērķa valsts valodās; produkti (un pavadošie materiāli) var nebūt izstrādāti saskaņā ar galamērķa valsts standartiem, specifikācijām un marķēšanas prasībām; un produkti var neatbilst galamērķa valsts spriegumam un citiem elektriskajiem standartiem (ja nepieciešams, nepieciešams adapteris vai pārveidotājs). Par to, lai produktu varētu likumīgi ievest galamērķa valstī, ir atbildīgs saņēmējs. Pasūtot no Ubuy vai tā saistītajiem uzņēmumiem, saņēmējs ir ierakstu importētājs, un viņam jāievēro visi galamērķa valsts tiesību akti un noteikumi.
Ne visi Ubuy uzskaitītie produkti ir pārdošanā, jo Ubuy ir globāla meklētājprogramma. Uz produktiem attiecas eksporta/tirdzniecības noteikumi.

€ 66

Pasūtiet tūlīt un saņemiet to Otrdiena, Augusts 25

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Funkcijas un priekšrocības

Contains 2M Tris, 1M Acetic acid with 0.05M EDTA
Inhibits metal dependent nucleases by chelating the divalent cations
Protects DNA during electrophoresis
Can be used for genomic and supercoiled DNA
Manufactured in an ISO 9001 and 13485 certified facility in the USA
For research use only, not for human or animal clinical/therapeutic use.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

50X TAE Buffer (Tris-Acetate-EDTA), 500 mL

€ 66

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Produkta informācija

Who Should Buy?

PRODUKTA APRAKSTS

About This Item

Klientu jautājumi un atbildes

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Pārskatiet šo produktu

Pros

Product Price History

Svarīga informācija

€ 66

Funkcijas un priekšrocības

Ubuy Assurance

Fast
Shipping

Free
Return^*