What is TBST TBS-Tween 20 used for?

TBST TBS-Tween 20 is commonly used in biological and biochemical applications, particularly in Western blotting and immunoassays. The Tween 20 acts as a surfactant, which helps reduce background noise and increase the specificity of the target detection by preventing non-specific binding. This makes it an ideal choice for preparing sample dilutions and wash buffers in various laboratory setups.

What are the components of TBST?

TBST stands for Tris-Buffered Saline with Tween 20. It typically includes Tris (a buffering agent), NaCl (sodium chloride), and Tween 20, which acts as a surfactant. The pH of 7.6 ensures the solution remains stable for biological reactions. The inherent properties of TBST make it effective for maintaining optimal conditions during experiments, especially when working with proteins and antibodies.

How should TBST TBS-Tween 20 be stored?

To maintain the integrity of TBST TBS-Tween 20, it should be stored at 2-8°C, away from light and direct heat sources. Additionally, ensure that the container is tightly sealed to avoid contamination. Proper storage ensures that the buffer remains effective for experimental use, facilitating accurate and reliable results in various biochemical analyses.

Can TBST TBS-Tween 20 be reused?

Reusing TBST TBS-Tween 20 is generally not recommended due to the potential for contamination and degradation of the buffer components. Each use might introduce residues that can interfere with subsequent experiments. Fresh preparation ensures the buffer's effectiveness and reliability, making it crucial for precise results in immunodetection applications.

What is the significance of the pH level in TBST?

The pH level of 7.6 in TBST is significant because it closely mimics physiological conditions, which is critical for maintaining protein structure and function during biochemical assays. A stable pH environment ensures effective binding of antibodies to target antigens, enhancing the accuracy of experimental outcomes. This pH balance is vital in labs conducting protein studies, sensitivity, and specificity of assays.

What are some alternatives to TBST TBS-Tween 20?

Alternatives to TBST TBS-Tween 20 include PBS (Phosphate Buffered Saline), and PBST (PBS with Tween 20). These buffers serve similar purposes but may have different ionic strengths or pH levels influencing assay performance. Choosing an alternative may depend on specific experimental requirements, such as the nature of the target protein or the type of assay being conducted.

What is the typical concentration of Tween 20 in TBST?

The typical concentration of Tween 20 in TBST is about 0.1% (v/v). This concentration is sufficient to reduce non-specific binding in assays while maintaining optimal binding conditions for antibodies. Tweaking the concentration can help fine-tune the assay's specificity, allowing researchers to achieve desired levels of sensitivity and specificity while working with various proteins.

Is TBST TBS-Tween 20 suitable for cell cultures?

While TBST TBS-Tween 20 is mainly used for biochemical assays, it can be used in certain cell culture applications, especially when washing cells or preparing samples for further analysis. However, it's essential to consider the application scope, as some cell types may be sensitive to surfactants. Careful optimization is advised to ensure cell viability and health during such experiments.

Can I prepare TBST TBS-Tween 20 in bulk?

Yes, you can prepare TBST TBS-Tween 20 in bulk, which is beneficial for laboratories requiring large volumes for repetitive assays. Bulk preparation involves mixing the specified amounts of Tris, NaCl, and Tween 20, followed by proper pH adjustment. Store the prepared solution under recommended conditions, ensuring consistent quality and effectiveness across different experimental runs.

Where can I buy TBST TBS-Tween 20, 10X, pH 7.6, 1000ml in Latvia?

You can buy TBST TBS-Tween 20, 10X, pH 7.6, 1000ml from Ubuy, which offers a variety of laboratory supplies, including this essential buffer solution. Ubuy ensures that you receive quality products, and their platform provides convenient navigation to help find the exact items you need for your laboratory applications.

Kā iepirkties TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit tiešsaistē no Ubuy?

Ir viegli iepirkties TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.

Vai TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.

Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

APEX Skatīt oriģinālu

TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit

Gentrox Liquid Buffers offer exceptional purity and reliability for your most critical research applications.

APEX

Preces #: 24667774

TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit

Preces #: 24667774

€ 116

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What Stands Out

Convenient Concentration

The 10X TBST solution offers a ready-to-use format, requiring only dilution for immediate application, saving time in lab preparations for researchers and scientists.

Optimal pH Level

Maintaining a stable pH of 7.6 ensures reliable performance and consistency in assays, making it ideal for sensitive biochemical experiments.

Generous Volume

Packaged in a 1000ml unit, this product provides ample supply for multiple experiments, reducing the frequency of reorders and optimizing lab budgets.

Produkta informācija

Container Type	Bottle
Item Form	Liquid
Volume	1000 ml
Brand Name	APEX
Manufacturer	Apex Bioresearch Products
Part Number	18-235B
Brand	APEX
Item Volume	1000 ml
Package Information	Bottle
Item Weight	2.2 lbs (1 kg)

Who Should Buy?

Laboratory Researchers
Ideal for researchers conducting immunoassays and protein studies using Western blotting and ELISA techniques.
Biotechnologists
Useful for biotechnologists who require a reliable buffer for stabilizing proteins during analytical procedures.
Quality Control Labs
Recommended for quality control laboratories needing a standardized reagent for verifying product consistency and efficacy.

Home Users
Not suitable for non-professionals or hobbyists lacking necessary background in handling biochemical reagents safely.

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jautājums: Kā iepirkties TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit tiešsaistē no Ubuy?

atbildi: Ir viegli iepirkties TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.
jautājums: Vai TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

atbildi: Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.
jautājums: Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

atbildi: Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

APEX Buffers Editorial Review

TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit is a high-quality liquid solution designed for various laboratory applications. Offered in a 1000 ml bottle, this product is manufactured by Apex Bioresearch Products and is praised for its reliability and effectiveness in providing consistent results. Customers have noted that the container's design ensures ease of use during experiments, and the specified pH level of 7.6 enhances its suitability for a diverse range of assays. Overall, this TBST solution stands out as a crucial reagent in the biochemistry and molecular biology fields, making it a go-to choice for researchers.

Customer Reviews & Ratings

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Pros

High-quality liquid solution
Convenient 1000 ml bottle storage
consistent pH level for experiments
Reliable for laboratory applications
Effective for various assays

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Some users may prefer smaller bottle sizes

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Ierobežojumi: attiecībā uz produktiem, kas tiek nosūtīti starptautiski, lūdzu, ņemiet vērā, ka jebkura ražotāja garantija var nebūt derīga; ražotāja servisa iespējas var nebūt pieejamas; produktu rokasgrāmatas, instrukcijas un drošības brīdinājumi var nebūt galamērķa valsts valodās; produkti (un pavadošie materiāli) var nebūt izstrādāti saskaņā ar galamērķa valsts standartiem, specifikācijām un marķēšanas prasībām; un produkti var neatbilst galamērķa valsts spriegumam un citiem elektriskajiem standartiem (ja nepieciešams, nepieciešams adapteris vai pārveidotājs). Par to, lai produktu varētu likumīgi ievest galamērķa valstī, ir atbildīgs saņēmējs. Pasūtot no Ubuy vai tā saistītajiem uzņēmumiem, saņēmējs ir ierakstu importētājs, un viņam jāievēro visi galamērķa valsts tiesību akti un noteikumi.
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€ 116

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Funkcijas un priekšrocības

Ultra pure cGMP grade chemicals ensure high quality.
4 fold purified water enhances buffer effectiveness.
Ideal for molecular biology research and protein characterization.
Purity > 99% guarantees reliable results.
Completely free of RNase, DNase, and endotoxins.
Perfect for genomics, proteomics, and diagnostic applications.

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TBST (TBS-Tween 20), 10X, pH 7.6, 1000ml/Unit

€ 116

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APEX Buffers Editorial Review

Customer Reviews & Ratings

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€ 116

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