What is Phosphate Buffered Saline (PBS), 10X?

Phosphate Buffered Saline (PBS), 10X is a buffer solution commonly used in biological research and cell culture. It maintains a stable pH and osmotic balance, making it ideal for diluting cells and biomolecules. Its formulation includes sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate, which together help keep cells viable during experiments. In practice, PBS is frequently used to rinse tissues, dilute samples, or prepare solutions, making it a staple in laboratories.

How do I prepare 1X solution from 10X PBS?

To prepare a 1X solution from 10X PBS, you simply need to dilute the 10X PBS with distilled water. Measure 1 part of the 10X PBS and mix it with 9 parts of distilled water. For example, if you use 100 mL of 10X PBS, add 900 mL of water to achieve a 1X concentration. This is essential for ensuring that the proper conditions are maintained for experiments and cell culture applications.

What are the common uses of 10X PBS in research?

10X PBS is widely used in various laboratory applications, including cell culture maintenance, washing cells post-trypsinization, and as a diluent for antibodies or drugs. Its isotonic nature prevents osmotic shock to cells, which is crucial when performing cell-based assays. Researchers also often use PBS for the preparation of buffers, maintaining pH stability, and conducting experiments that require a controlled ionic environment.

Is 10X PBS suitable for sensitive biological assays?

Yes, 10X PBS is suitable for sensitive biological assays, provided it's prepared properly to 1X concentration. Its ability to maintain pH and osmotic balance makes it a reliable choice for a variety of assays, including ELISA, Western blotting, and flow cytometry. Additionally, the balanced ionic composition minimizes interference, helping to ensure accurate results in sensitive applications.

What are the storage conditions for PBS?

PBS should be stored at room temperature or refrigerated if unopened. Once opened, it can be stored at 4°C and used within a certain period depending on contamination risks. Always ensure the container is well-sealed to prevent contamination, which might compromise results in experiments. Proper storage extends the usable life of PBS, making it a reliable buffer for long-term projects.

Can I use PBS in mammalian cell culture?

Absolutely! PBS is widely utilized in mammalian cell culture environments. It is commonly employed for washing cells, re-suspending cells, or diluting compounds. Using PBS minimizes the risk of osmotic shock, which is critical for maintaining cell viability. Whether you are passaging cells, performing transfections, or preparing samples for microscopy, PBS serves as an effective buffer during these processes.

What precautions should I take when using PBS?

While PBS is generally safe to use, maintaining sterility is crucial, especially when working with cell cultures. Use aseptic techniques while handling PBS to avoid contamination. Additionally, always double-check the buffer's pH before use to ensure it suits your specific application and prevents unwanted reactions in sensitive assays, thus improving the reliability of your experimental outcomes.

Is it possible to make my own PBS?

Yes, creating your own PBS solution is possible, but it involves precise measurements and sterilization methods to ensure the buffer's effectiveness. By combining sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate with distilled water, you can formulate your own. However, using pre-made PBS from reputable suppliers is often more convenient and ensures reliable results in laboratory settings.

Where can I buy Phosphate Buffered Saline (PBS), 10X in Latvia?

You can purchase Phosphate Buffered Saline (PBS), 10X from Ubuy in Latvia. Ubuy offers a wide range of laboratory supplies, including high-quality PBS, making it convenient for researchers and laboratories to stock their essential reagents online. With reliable shipping and excellent customer service, Ubuy can be your go-to source for PBS and other laboratory needs.

What is the pH of 10X PBS?

The pH of 10X PBS is typically around 7.4, which is crucial for maintaining physiological conditions in biological experiments. Ensuring that the PBS is at this pH level helps to better support cell and tissue viability during assays. Always check the pH prior to use, especially when making dilutions, to guarantee accuracy in your experimental setups and maintain consistency in results.

Kā iepirkties Phosphate buffered Saline (PBS), 10X tiešsaistē no Ubuy?

Ir viegli iepirkties Phosphate buffered Saline (PBS), 10X tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.

Vai Phosphate buffered Saline (PBS), 10X ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. Phosphate buffered Saline (PBS), 10X nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.

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Phosphate buffered saline (PBS) is a widely used solution for various purposes. Our PBS is produced in a cGMP-compliant facility and is rigorously tested for quality.

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Phosphate buffered Saline (PBS), 10X

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Concentration Strength

This 10X phosphate buffered saline offers a higher concentration, ensuring effective dilution for various laboratory applications, enhancing reliability in biochemical assays and cellular experiments.

pH Stability

Formulated to maintain a stable pH, this PBS ensures optimal conditions for cellular and molecular biology experiments, reducing variability and increasing reproducibility in research results.

Versatile Application

Designed for various applications in cell culture, molecular biology, and immunology, this PBS provides researchers with a reliable solution for buffer needs, promoting efficiency and performance in laboratory protocols.

Produkta informācija

Container Type	Bottle
Item Form	Liquid
Volume	500 ml
Brand Name	Hygia Reagents
Manufacturer	Hygia Reagents
UPC	755457565939
Included Components	1 bottle
Part Number	HB-PBS10
Brand	Hygia Reagents
Item Volume	500 ml
Package Information	Bottle
Item Weight	1 lbs (450 grams)

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Cell Culture
Ideal for maintaining pH and osmolarity in cell culture applications and cellular biology research.
Molecular Biology
Useful for diluting and washing biomolecules during molecular biology experiments and assays.
Laboratory Use
Essential for preparing buffer solutions in various laboratory experiments requiring stable pH conditions.

Clinical Use
Not suitable for direct medical applications or as a treatment due to lack of sterility verification.

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Phosphate buffered Saline (PBS), 10X

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jautājums: Kā iepirkties Phosphate buffered Saline (PBS), 10X tiešsaistē no Ubuy?

atbildi: Ir viegli iepirkties Phosphate buffered Saline (PBS), 10X tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.
jautājums: Vai Phosphate buffered Saline (PBS), 10X ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

atbildi: Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. Phosphate buffered Saline (PBS), 10X nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.
jautājums: Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

atbildi: Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

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Pros

Highly effective buffer solution
Stable and reliable formula
Versatile for multiple applications
Easy to prepare and use
Excellent for cell culture

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May require additional storage space.

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Funkcijas un priekšrocības

Phosphate buffered saline (PBS) is a versatile solution for various purposes.
Our PBS is produced in a cGMP-compliant facility.
Filtered through a 0.1μm sterile filter before filling.
Rigorous quality testing done, and each lot is tested for sterility, pH, osmolality, and endotoxin.
Available in 500mL and concentrated forms.
Formulated without calcium and magnesium.

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