What is Citrate Buffer 0.1M used for?

Citrate Buffer 0.1M is primarily used in biochemical and molecular biology applications, including enzyme assays and electrophoresis. This buffer helps maintain a stable pH environment for various biological reactions, which is crucial for accurate experimental results. For example, researchers using this buffer can carry out protein electrophoresis without pH fluctuations that could affect the migration of proteins.

Why is it important to maintain a specific pH when using Citrate Buffer?

Maintaining a specific pH, especially in the range of 4.6 to 6.2 for Citrate Buffer, is critical for enzyme activity and stability. Many enzymes require a precise pH to function optimally, and deviations can lead to denaturation or inactivation. For scientists in enzymology, using this buffer ensures that enzymatic reactions occur under ideal conditions, providing reliable data and reproducibility in experiments.

How should Citrate Buffer 0.1M be stored?

Citrate Buffer 0.1M should be stored at room temperature in a tightly sealed container to prevent contamination. Avoid exposure to direct sunlight or extreme temperatures to maintain pH stability. For use in laboratory settings, proper storage ensures that the buffer retains its effectiveness for applications such as cell culture or molecular analyses, where pH consistency is paramount.

Can I use Citrate Buffer 0.1M for cell culture?

Yes, Citrate Buffer 0.1M can be used in specific cell culture applications, although it’s generally more common in biochemical experiments. It can help maintain appropriate pH levels when culturing sensitive cell lines. Researchers often rely on such buffers when performing assays where the pH must be strictly regulated to prevent cellular stress and ensure optimal cell growth during experiments.

What are the main components of Citrate Buffer?

Citrate Buffer is primarily composed of citric acid and its corresponding salts, such as sodium citrate. These components allow for effective buffering capacity within the specified pH range. The interplay between citric acid and its conjugate base stabilizes the pH during chemical reactions, making it a reliable choice for various laboratory applications where precise pH control is essential.

Is Citrate Buffer 0.1M suitable for PCR amplification?

Citrate Buffer 0.1M is generally not the first choice for PCR amplification, as PCR typically requires a more neutral pH for optimal enzyme function. However, it can be useful in specific pre-PCR preparations or certain protocols where maintaining a slightly acidic environment is necessary. Always refer to the specific protocol details or literature for best practices when using buffers in PCR.

What techniques can benefit from using Citrate Buffer?

Techniques such as gel electrophoresis, enzyme assays, and immunoassays can greatly benefit from using Citrate Buffer. The ability to maintain a consistent pH helps ensure that reactions proceed without interference. For example, in protein gel electrophoresis, using the correct buffer allows for accurate separation based on charge and size, leading to clearer results and more reliable analysis.

Can Citrate Buffer be prepared fresh, or is it better to buy it ready-made?

Both options are valid; Citrate Buffer can be prepared fresh based on specific concentrations and pH requirements, allowing for customization per protocol needs. However, purchasing ready-made Citrate Buffer ensures consistent quality and saves time in routine laboratory work. For researchers conducting multiple experiments, ready-made options streamline processes while maintaining reliability and reproducibility in results.

Is Citrate Buffer 0.1M compatible with other buffers?

Citrate Buffer 0.1M can be compatible with other buffers; however, mixing them should be approached with caution as it may lead to unexpected pH changes. Researchers often choose to use citrate as a standalone buffer to ensure optimal performance in enzymatic reactions or biochemical assays. It’s best suited to applications that specifically require its buffering properties without complicating the reaction conditions.

Where can I buy Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL?

You can buy Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL from Ubuy. Ubuy offers a wide range of laboratory supplies, ensuring that you have access to quality products for your scientific needs.

Kā iepirkties Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL tiešsaistē no Ubuy?

Ir viegli iepirkties Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.

Vai Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.

Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

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We offer a comprehensive range of biological buffers for use in research, diagnostics, drug development, and manufacturing.

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Preces #: 79978951

Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

Preces #: 79978951

€ 44

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What Stands Out

Precise pH Range

Maintains a stable pH level between 4.6 and 6.2, ensuring accurate results in various laboratory experiments and applications.

Large Volume

Supply of 500 mL is ideal for extended usage in multiple experiments, reducing the need for frequent reordering and boosting efficiency.

High Purity

Formulated with high-grade chemicals, guaranteeing minimal contaminants for reliable and reproducible results in sensitive scientific research.

Produkta informācija

Manufactured in USA under ISO 9001 and 13485 certified facility
For research use only, not for human or animal clinical/therapeutic use
Each production lot undergoes rigorous quality control tests in certified 14644 clean room conditions
Ideal for RNA isolation and antigen detection in fixation medium
Popular choice for immunofluorescent staining applications
Comprehensive range of biological buffers for research, diagnostics, and drug development

Item Form	Liquid
Item Weight	12 ounces (340.2 grams)
Volume	500 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.129
Brand	eCon Lab Supply
Item Volume	500 ml

Who Should Buy?

Laboratory Scientists
Ideal for scientists conducting experiments requiring stable pH levels in biochemical and molecular biology applications.
Educational Institutions
Useful for schools and universities teaching laboratory techniques in chemistry and biology involving pH buffers.
Pharmaceutical Research
Essential for researchers working on drug formulations where precise pH maintenance is crucial for stability and efficacy.

General Home Use
Not suitable for household applications, as it is a specialized laboratory chemical not typically needed at home.

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Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

About This Item

Looking for a reliable citrate buffer for your biochemical applications? Look no further than our 0.1M Citrate Buffer, offering a pH range of 4.6-6.2. With a generous volume of 500 mL, this high-quality citrate buffer is perfect for your lab needs. Whether you are conducting research or carrying out experiments, our citrate buffer ensures consistent and accurate results.

Buy online now and experience the best in citrate buffering solutions.

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jautājums: Kā iepirkties Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL tiešsaistē no Ubuy?

atbildi: Ir viegli iepirkties Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.
jautājums: Vai Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

atbildi: Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.
jautājums: Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

atbildi: Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

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Accurate pH range (4.6-6.2)
Convenient 500 mL size
Excellent for biochemical applications
Stable formulation and long shelf life
Compatible with various assays

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Limited use outside of pH range

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€ 44

Pasūtiet tūlīt un saņemiet to Ceturtdiena, Septembris 10

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Funkcijas un priekšrocības

Comprehensive range of biological buffers
Suitable for research, diagnostics, drug development, and manufacturing
Manufactured in an ISO 9001 and 13485 certified facility
Each production lot manufactured under stringent quality control tests
Citrate buffers prevent base hydrolysis and break cross-links between antigens
Popular choice for immunofluorescent staining applications

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Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Citrate Buffer 0.1M, pH 4.6-6.2, 500 mL

€ 44

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What Stands Out

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Who Should Buy?

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About This Item

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eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

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Product Price History

Svarīga informācija

€ 44

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