What is Citrate Buffer, 1M, pH 6.0 used for?

Citrate Buffer, 1M, pH 6.0, is primarily utilized in biochemical applications, particularly in molecular biology and biochemistry. It acts as a stabilizing agent that maintains pH conditions during various experiments, including enzyme reactions and protein assays. This buffer is essential for ensuring consistent results in DNA and RNA studies, as many assays require a specific pH for optimal performance.

How do you prepare Citrate Buffer, 1M, pH 6.0?

To prepare Citrate Buffer, 1M, pH 6.0, mix 1M citric acid and sodium citrate at a ratio that achieves the desired pH level. Typically, this involves combining 0.1M citric acid and 0.1M sodium citrate in appropriate proportions and adjusting the pH using a pH meter. Proper preparation is crucial for obtaining accurate results in your experiments, particularly when testing acids or bases, or calibrating pH-sensitive equipment.

Can I use Citrate Buffer for cell culture applications?

Citrate Buffer, 1M, pH 6.0 is not typically used directly in cell culture applications, but it can be beneficial for specific experimental setups that require a stable pH environment. For example, researchers might use it for washing cells or preparing reagents where pH stabilization is crucial. Always consider the specific requirements of your cell line before application, as pH sensitivity varies across different cell types.

Is Citrate Buffer, 1M, pH 6.0 suitable for protein analyses?

Yes, Citrate Buffer, 1M, pH 6.0 is ideal for protein analyses, particularly in assays involving enzyme activity and protein stability. Many proteins function optimally at a pH around 6.0, making this buffer perfect for maintaining conditions that are conducive to accurate measurements. This buffer helps minimize fluctuations that could impact enzyme kinetics or structural integrity during analysis.

What are the storage requirements for Citrate Buffer, 1M, pH 6.0?

Citrate Buffer, 1M, pH 6.0 should be stored in a cool, dry place, away from direct sunlight, to maintain its stability. Ideally, it should be stored at room temperature, in a tightly sealed container to prevent contamination. Proper storage is essential because it ensures the buffer remains effective for its intended applications, which may involve metabolic or chemical reactions sensitive to environmental changes.

Can Citrate Buffer be used in clinical laboratories?

Yes, Citrate Buffer, 1M, pH 6.0 finds application in clinical laboratories, particularly in diagnostic assays where maintaining a specific pH is vital for accuracy. It is often used in coagulation studies and sample preparations where pH can affect the behavior of various biomolecules. Its reliable performance makes it a staple in routine laboratory practices.

What precautions should I take when using Citrate Buffer?

When using Citrate Buffer, 1M, pH 6.0, it is essential to wear appropriate PPE, including gloves and goggles, to protect against potential splashes. Additionally, ensure that your workspace is clean and free from contaminants that could affect experimental results. Practicing good laboratory hygiene is critical to maintaining the integrity of your experiments and avoiding cross-contamination.

Is Citrate Buffer compatible with other buffer systems?

Citrate Buffer, 1M, pH 6.0 can be compatible with certain other buffer systems depending on the specific requirements of your application. For example, it may be mixed with phosphate buffers in certain scenarios to achieve a desired pH or ionic strength. However, care must be taken, as combining buffers can lead to unpredictable results; it’s pivotal to test compatibility for your specific experimental conditions.

What is the role of pH in experiments using Citrate Buffer?

In experiments involving Citrate Buffer, pH plays a crucial role in influencing molecular interactions and enzyme activity. Many biochemical processes are pH-dependent; thus, maintaining a stable pH at 6.0 is essential for achieving reproducibility in experimental results. It's particularly relevant in enzyme assays where deviations from optimal pH can lead to altered reaction rates or product formation.

Where can I buy Citrate Buffer, 1M, pH 6.0, 250 mL in Latvia?

You can purchase Citrate Buffer, 1M, pH 6.0, 250 mL from Ubuy in Latvia. Ubuy provides a reliable platform for sourcing quality laboratory reagents, including this essential buffer. By shopping there, you can ensure that you are getting a product that meets high standards for your experimental and research needs.

Kā iepirkties Citrate Buffer, 1M, (pH 6.0), 250 mL tiešsaistē no Ubuy?

Ir viegli iepirkties Citrate Buffer, 1M, (pH 6.0), 250 mL tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.

Vai Citrate Buffer, 1M, (pH 6.0), 250 mL ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. Citrate Buffer, 1M, (pH 6.0), 250 mL nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.

Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

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Citrate Buffer, 1M, (pH 6.0), 250 mL

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Preces #: 84061248

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What Stands Out

Optimal pH Control

Ensures reliable results in experiments by maintaining a stable pH of 6.0, critical for biochemical and molecular applications.

High Concentration

This 1M concentration offers enhanced buffer capacity, effectively stabilizing pH during reactions and providing robust support for various laboratory applications.

Convenient Volume

With a 250 mL volume, this buffer provides ample supply for multiple experiments, reducing the need for frequent reordering and optimizing lab efficiency.

Produkta informācija

Item Form	Liquid
Item Weight	4 ounces (113.4 grams)
Volume	250 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.133
Brand	eCon Lab Supply
Item Volume	250 ml

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Who Should Buy?

Biochemical Research
Ideal for laboratories conducting enzyme assays and other biochemical reactions requiring a stable pH environment.
Molecular Biology
Beneficial for molecular biologists needing a reliable buffer for DNA/RNA purification and manipulation.
Pharmaceutical Applications
Useful in the pharmaceutical industry for drug formulation and stability testing that requires specific pH conditions.

High-Temperature Experiments
Not suitable for experiments requiring high temperatures, as buffer stability may be compromised.

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jautājums: Kā iepirkties Citrate Buffer, 1M, (pH 6.0), 250 mL tiešsaistē no Ubuy?

atbildi: Ir viegli iepirkties Citrate Buffer, 1M, (pH 6.0), 250 mL tiešsaistē no Ubuy.. Jums vienkārši ir jāmeklē prece, izrakstīšanās laikā jāizvēlas piegādes veids un jāsaņem tā piegāde uz jūsu atrašanās vietu.
jautājums: Vai Citrate Buffer, 1M, (pH 6.0), 250 mL ir pieejams iepirkšanās tiešsaistē šeit: Latvia?

atbildi: Jā, Ubuy Latvia šis produkts ir pieejams, lai jūs varētu iepirkties par saprātīgu cenu.. Citrate Buffer, 1M, (pH 6.0), 250 mL nav pieejams lokāli, taču varat mums uzticēt mūsu ātrās piegādes pakalpojumus.
jautājums: Cik ilgs laiks nepieciešams, lai saņemtu preci pēc pasūtījuma veikšanas?

atbildi: Jūsu pasūtītās preces piegādes laiks atšķiras atkarībā no jūsu pasūtījuma un jūsu izvēlētā piegādes veida.. Paredzamais piegādes laiks tiek minēts norēķināšanās procesā, tāpēc iepērkoties esiet bezrūpīgs.

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Pros

Highly effective pH stabilizer
Easy to use and measure
Compatible with various applications
Reliable performance across experiments
Well-packaged for safe handling

Mīnusi

Occasional sediment formation

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Ierobežojumi: attiecībā uz produktiem, kas tiek nosūtīti starptautiski, lūdzu, ņemiet vērā, ka jebkura ražotāja garantija var nebūt derīga; ražotāja servisa iespējas var nebūt pieejamas; produktu rokasgrāmatas, instrukcijas un drošības brīdinājumi var nebūt galamērķa valsts valodās; produkti (un pavadošie materiāli) var nebūt izstrādāti saskaņā ar galamērķa valsts standartiem, specifikācijām un marķēšanas prasībām; un produkti var neatbilst galamērķa valsts spriegumam un citiem elektriskajiem standartiem (ja nepieciešams, nepieciešams adapteris vai pārveidotājs). Par to, lai produktu varētu likumīgi ievest galamērķa valstī, ir atbildīgs saņēmējs. Pasūtot no Ubuy vai tā saistītajiem uzņēmumiem, saņēmējs ir ierakstu importētājs, un viņam jāievēro visi galamērķa valsts tiesību akti un noteikumi.
Ne visi Ubuy uzskaitītie produkti ir pārdošanā, jo Ubuy ir globāla meklētājprogramma. Uz produktiem attiecas eksporta/tirdzniecības noteikumi.

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Funkcijas un priekšrocības

Comprehensive range of biological buffers
Buffers important for protein research and molecular biology
Manufactured under stringent Good Manufacturing Practices
Citrate buffer's versatile applications in RNA isolation and immunofluorescent staining
Popular choice for biological research and clinical applications
For research use only. Not for human or animal clinical/therapeutic use

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Citrate Buffer, 1M, (pH 6.0), 250 mL

€ 60

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Who Should Buy?

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eCon Lab Supply Buffers Editorial Review

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